Huang Y, Mou SF, Riviello JM. This information collected in Table 2 is primarily a comprehensive review of information about inorganic and organic forms of phosphorus present in organs and tissues of different plant species. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Int Rev Hydrobiol. Currently, nuclear magnetic resonance spectroscopy (NMR spectroscopy) is a versatile technique that supplies information about the integration and regulation of metabolic pathways of plants through a combination of in vivo and in vitro measurements. Direct analysis of plant materials and comparison of extraction processes using ICP-AES. Privacy It should also be noted that especially a high ion content may influence the obtained results. If you are the author of this article, you do not need to request permission to reproduce figures Majed N, Li Y, Gu AZ. Nowadays, obviously being aware of higher limit of detection, nuclear magnetic resonance spectroscopy, especially the 31P NMR technique, is thought to be the most universal analytical tool allowing to determine various chemical forms of plant phosphorus qualitatively and quantitatively, at the same time. Orthophosphate, phytate, and total phosphorus determination in cereals by flow injection analysis. Most methods for phosphate determination are based on the spectrophotometric detection of a coloured phosphomolybdate complex [36]. Resano M, Briceo J, Belarra MA. of phosphorus) + No dist. (20 gm. Dorota Wieczorek. Cai H, Chuang WG, Cui X, Cheng RH, Chiu K, Chen Z, Ding S. High Resolution (31)P NMR Spectroscopy Generates a Quantitative Evolution Profile of Phosphorous Translocation in Germinating Sesame Seed. New insight into the role of trehalose-6-phosphate. Even if more advanced spectrophotometric measurements and classical variants of absorption (FAAS) or emission (ICP-AES/ ICP-OES) spectrometry techniques are used, it is necessary at first to isolate various forms of phosphorus from the matrix, and then to mineralize them prior the determination. 1985;77:13641. Ullrich-Eberius CI, Novacky A, van Bel AJ. Estimation of phosphorus - Equation, Procedure, Calculation, Example This complex is reduced to an intensely blue-colored complex by ascorbic acid. Interestingly, illumination and hormones do not affect the final residual concentration of phytate. A large group of phosphoester nucleoside derivatives (e.g., ATP, ADP, UDP) is a specific primary reservoir of energy distributed in phosphorylation and dephosphorylation reactions of other chemical compounds. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Although the total phosphorus content in soil can be relatively high, the acquisition of this element by plants is often limited because inorganic phosphates are characterized by low solubility and a high sorption capacity in soil [4]. The work of phosphorus estimation was initiated by Neumann . 10 n sulphuric acid. Van Veldhoven PP, Mannaerts GP. provided correct acknowledgement is given. Front Microbiol. Among them, spectrophotometric methods based on molybdenum blue colouration are still the most widely used. Nevertheless, NMR spectroscopy is thought to be nondestructive, noninvasive, nonbiased, easily quantifiable, and requires few or no extra steps for sample preparation or fractionation. Additionally, some plant components, such as nucleotides, can also give elevated values of InsP6 [64]. Modification of the phosphate group by the addition of choline, serine, ethanoloamine, inositol or glycerol leads to the formation of structurally different classes of phospholipids referred successively to as phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, phosphatidylinositol or phosphatidylglycerol [9]. The proposed changes include extending the extraction time to 16h, lowering the centrifugation temperature to 10C and adding a matrix cleaning step to improve phytate recovery. of an ammonium molybdate and sulphuric acid solution and then of l c.c. Both of these techniques are based on elemental analysis of the surface of a sample bombarded by photons or ion beams. The molybdenum blue reaction for the determination of orthophosphate revisited: opening the black box. Google Scholar. (40 gm. 1995;52:3215. 1994;47:1929. Opportunities for improving phosphorus-use efficiency in crop plants. Because InsP3-InsP5 pure standards are needed in most methods, accurate purity information is usually lacking, even when using similar treatment methods, the results obtained in different studies are conflicting [68]. This cookie is set by GDPR Cookie Consent plugin. 2.2Ammonium molybdate and antimony potassium tartrate react in an acid medium with dilute solutions of phosphorus to form an antimony-phospho-molybdate complex. ESTIMATION OF PHOSPHORUS: MOLYBDIC ACID-STANNOUS CHLORIDE REAGENT. Although phytates are not the one or even the crucial form of organic phosphorus in plant tissues, the vast majority of analytical reports are addressed to this fraction. 1. Appl Environ Microbiol. Determination of phosphorus compounds in plant tissues: from colourimetry to advanced instrumental analytical chemistry, https://doi.org/10.1186/s13007-022-00854-6, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. TP is the sum of the contents of all P-containing compounds in the plant sample. They found that the metabolism of phosphorus was temperature-dependent and under the influence of illumination and hormones. Electrothermal atomic absorption spectrometry, High-performance anion-exchange chromatography, High-resolution continuum source electrothermal atomic absorption spectrometry, Ion pair chromatography high-performance ion chromatography (HPIC), Inductively coupled plasma atomic emission spectrometry or inductively coupled plasma optical emission spectrometry, Inductively coupled plasma mass spectrometry, Line source flame atomic absorption spectrometry. Using the IPC procedures developed by [69], it is possible to separate and quantitatively determine InsP3 to InsP6 based only on the number of phosphate groups in the myo-inositol ring without differentiating isomeric forms of InsP6. In plant tissues, phosphorus occurs in organic forms such as phosphate monoester (C-O-P bond) (e.g., phytate), phosphate diesters (COPOC bond) (e.g., nucleotides and phospholipids), and inorganic forms such as orthophosphate, pyrophosphate (P-O-P bond), and polyphosphate. A simple spectrophotometric method for the determination of - PubMed New Phytol. . 2002;956:6570. J Chromatogr A. Anal Chim Acta. Organophosphorus compounds occurring in living organisms are mostly organic phosphate esters, in which phosphate groups are bound to organic units by a C-O-P linkage. After correlation with a standard curve of phytic acid, the value obtained should be multiplied by 0.282 (molar ratio of P in the IP6 molecule) to express the content of Pphy in the plant sample [61]. A modified single solution method for the determination of phosphate in natural waters. The role of phospholipids in plasma membrane ATPase Activity in Vigna radiata L. (Mung Bean) Roots and Hypocotyls. The results indicate that all bracken (blade stipe rhizome) and bluebell parts (leaves scapes flowers roots bulbs seeds) contained a significant percentage of Pi in the form of orthophosphate. Roux SJ, Steinebrunner I. Extracellular ATP: an unexpected role as a signaler in plants. Determination of phytic acid and inositol pentakisphosphates in foods by high-performance ion chromatography. 1995; 49:245258. Anal Chem. In recent decades, 31P NMR spectroscopy has been widely used to simultaneously identify and quantify all phosphorus-containing compounds. Expression analyses of three members of the AtPHO1 family reveal differential interactions between signaling pathways involved in phosphate deficiency and the responses to auxin, cytokinin, and abscisic acid. Test tubes, pipettes, beakers, etc. water. Ebuele VO, Santoro A, Thoss V. Characterization of Plant Seeds by Phosphorus-31 Nuclear Magnetic Resonance Spectroscopy. 2020;102:50716. of total phosphorus as described by Bartlett (6) : "The phosphorus heating method could not be used to assay inorganic orthophosphate in . Furthermore, many XRF analysers require relatively high amounts of plant material (approximately 1 to 10g) for analysis [24]. Food Eng Prog. Sandberg AS, Ahderinne R. HPLC Method for Determination of inositol Tri-, Tetra-, Penta-, and Hexaphosphates in Foods and Intestinal Contents. Additionally, the use of electrothermal atomic absorption spectrometry (ETAAS), and more specifically high resolution-continuum source ET spectrometry, thanks to the judicious selection of the chemical modifier and the temperature program, permits the direct determination of phosphorus in plant tissues by monitoring both atomic and molecular absorption spectrometry in the vicinity of the 213.618nm line. 1985;16:787800. Plant Methods Carvalho Vieira E, Nogueira ARA. What is the easiest way to analyse the phosphorus in - ResearchGate Probing plant metabolism with NMR. Food Chem. However, the nature of the NMR measurements required for these research tasks, particularly regarding the hardware requirements, the detection scheme, and the sensitivity of the analysis, is extremely variable [77]. Necessary cookies are absolutely essential for the website to function properly. Kwanyuen P, Burton JW. Ruiz-Calero V, Galceran MT. water taken in the experimental tube. NMR signals are characterized by their frequency (chemical shift), intensity, fine structure, and magnetic relaxation properties, all of which reflect the precise environment of the detected nucleus. The molybdenum blue reaction for the determination of orthophosphate 2015;257258:10214. J Agric Food Chem. Determination of phosphorus compounds in plant tissues: from Experimental Reagents. 2015;19:1616. Nagul EA, McKelvie ID, Worsfold P, Kolev SD. of phosphate in the unknown sample can be estimated. The acetic acid phosphorus extraction method which has the best correlation with the Olsen method was further simplified and its significant correlation with the Olsen method was 0.855. In this case, the phosphate released from phytic acid via enzymatic dephosphorylation was measured using a modified colourimetric molybdenum blue assay and calculated as the TP or phytic acid content of the original sample. Anal Bioanal Chem. Polyphosphates are linear polymers of inorganic phosphate (Pi) units with sizes ranging from tripolyphosphate (three Pi units) to long-chain polyP (approximately 1000 Pi units) linked by phosphoanhydride bonds. The absorbance of the resulting complex was measured with a spectrophotometer at 880nm [39]. Burbano C, Muzquiz M, Osagie A, Ayet G, Cuadrado C. Determination of phytate and lower inositol phosphates in Spanish legumes by HPLC methodology. Read more about how to correctly acknowledge RSC content. Simple and Sensitive Spectrophotometric Method for Phytic Acid Determination in Grains. TP can be determined by various spectrophotometric (molybdenum blue colourimetric method, malachite green assay) or spectrometric methods (FAAS, ETAAS, ICP-OES, ICP-AES or ICP-MS) [18] (Table 1). Moore KL, Lombi E, Zhao FJ, Grovenor CRM. In a continued study, cumin, fennel, flax, mustard, poppy, and sesame seeds were analysed for P species by the same group [94]. Fetching data from CrossRef. Phytate was the dominant P species in seeds and constituted almost half of the total P in chaff but was only detected in minor amounts in stem residue. Phosphate acquisition. 1962;27:316. 2009;60:96778. Planta. 1984;161:4652. 2016;99:73843. Welcome to BiologyDiscussion! Extractable Phosphorus - Olsen Method - UC Davis The calibration curve was formed using concentrations of 0.5-3.0 mg/L. Ion chromatographic separations of phosphorus species: A review. Based on our laboratory experience and according to the systematic review presented hereby, it may be concluded that each analytical problem related to the determination of phosphorus requires an individual approach and should be dedicated to a defined problem. (10 gm. Chen Q. Misson J, Henner P, Morello M, Floriani M, Wu TD, Guerquin-Kern JL, Fvrier L. Use of phosphate to avoid uranium toxicity in Arabidopsis thaliana leads to alterations of morphological and physiological responses regulated by phosphate availability. The authors concluded that exhaustive analysis of phosphorus metabolites in seeds requires a minimum of four extracts, which means an aqueous, acidic, nucleotide, and phospholipid extraction procedure. 2008;227:102536. Some important soil properties that influence the solubility of phosphorus compounds are pH, the concentrations of iron, aluminium, calcium ions and the nature of the soil particles, mainly their surface area [2]. Method 300.1 Determination of inorganic anions in drinking water by ion chromatography, 1997. By changing the physical properties of membranes, they increase or decrease the activity of transport proteins (i.e., membrane ATPase) and thereby help regulate membrane transport. Determination of phytic acid in foods by ion chromatography with post-column derivatization. In this article we will discuss about the colorimetric method for estimation of phosphorus in plants. Department of Pharmacy and Ecological Chemistry, Institute of Chemistry, University of Opole, Oleska 48, 45-052, Opole, Poland, Dorota Wieczorek,Beata yszka-Haberecht,Anna Kafka&Jacek Lipok, You can also search for this author in
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