The variability in the manual processes that impact reproducibility, quantitation, time to result, and overall data reliability is eliminated. Automation of protein separation and immunodetection eliminates many of the tedious, error-prone steps of traditional Western blotting which limit data quality. With Jess, it's pipette, run & done! Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Multiplex with chemiluminescence & fluorescence channels. Get the latest news, product updates, and promotions: The product is shipped at ambient temperature. Why bother stripping and reprobing? Bio-Techne uses cookies to provide you with a great website experience. ", Melyssa Bratton, Ph.D., Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded. Even better, shell quantitate the results for you too. Image courtesy of ProteinSimple. ", Noelle Anastasio, Ph.D., Nuffield Department of Medicine, Target Discovery Institute, "Protein analysis using [Simple Western] can be performed faster, eliminating tedious work and saving time. For samples containing 0.005 - 0.2 mg/mL of protein, chemiluminescence total protein assay methods can get you accurate normalized immunoassay data without having to rely on variable housekeeping proteins. Which Brands are Currently Available on biotechne.com? Forbes D. Porter. Everything happens inside a capillary, and Jess precisely controls the protein separation and reagent additions and incubations, even the detection. Protein analysis comes with many challenges, labor intensive protocols, increase time to result, and multiple hands-on steps increase user error and data variability. Copyright 2023 R&D Systems, Inc. All Rights Reserved. Centre for Tissue Engineering & Regenerative Medicine, University Kebangsaan Malaysia (UKM) Medical Centre, "Jess is a user-friendly, multi-purpose platform that gives me the possibility to perform Western blot analysis on precious samples, as well as quantifying proteins expressed in the cells at the same time. Scan to launch video protocol 2021 ProteinSimple. FAX 612 656 4400. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently. Texas A&M Institute for Genome Sciences and Society. Searching for key inflammation molecules", Kirstine Nolling Jensen, PhD Student, Simple Western immunoassays take place in a capillary. Learn more about this offer atSimple Western Antibody Submission Promotion. By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We empower you to make your next discovery by eliminating common protein analysis workflow challenges. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE and how expensive is. Jess, she's like western blot meets ELISA in one. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. x][SH~'Pbo4BuJ}&&,gzyc,[4,I]%&}6b-YYyEU^o'WlM9Ulz|pY1]M?$}x/>TI Whilst. Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool. It would take about a week to run 40 proteins using Western blotting. The chemiluminescent reaction is recorded by a CCD camera in a series of images over time. Or look at multiple targets and parameters simultaneously. Sample preparation, separation, and immobilization for theChemiluminescent Total Protein Assayare exactly the same asSimple Western automated immunoassays. Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. Just load your samples and reagents into the microplate, and Jess does the rest. She separates your protein by size and precisely manages antibody additions, incubations, washes, and even the detection steps. Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, View all Automated Western Blot Instrumentation FAQs. ProteinSimple branded instruments are available to quote. Snap! Reduce your run costs and labor costcompared to traditional Western blot with automatedSimple Western. 1 0 obj Toll-free 800 343 7475 For chemiluminescent detection, samples are first incubated with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. We will not share your information for any other purposes. ", Laura Martins, Ph.D., And obviously I like getting results the same day rather than having to wait two days", Emma Wilson (ne Humphrey), Ph.D., Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Senior Research Scientist, TCG Lifesciences, "Jess allows me to reduce the preparation time, save on reagents, samples, and antibodies, while allowing easier optimization of target proteins/antibodies. endobj At best, you end up with Western blot-like, semi-quantitative results when what you really need, and deserve, is highly reproducible immunoassay quantitation. Jess gives you four different ways to analyze proteins: What does the data look like? New Assay>Jess-Immunoassay: Fluorescence; Size Range:12-230 kDa; Cartridge: 25. And with in-built protein normalization, you'll have the confidence you need in your results, experiment after experiment. Find protocols for special samples as well as specific applications. PL3-0004 RevC Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 info@proteinsimple.com proteinsimple.com ANTIBODIES ProteinSimple branded instruments are available to quote. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. All assay steps from protein separation, immunoprobing, detection, and analysis are fully automated. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy. Detection of Stat3 (green) and phospho-Stat3 (red) in K562 cell lysate. Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module! With Jesss chemiluminescent detection youll get picogram-level sensitivity, letting you maximize the data you get from your sample. This 15-minute Tech Talk focuses on Adeno-associated virus (AAV) Empty and Full separation using the imaged cIEF method with the Maurice system. I need to do protein normalization ", Debbie Hicks, Ph.D., Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics, "The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. ProteinSimple branded instruments are available to quote. All assay steps from protein separation, immunoprobing, detection and analysis of data are fully automated. ", Jon Voutila, Ph.D., ProteinSimple: Transferring your Traditional Western Blot to Wes Next, your protein lysate is loaded, and capillaries are lowered to make contact with running buffer. Come back to fully analyzed western blotting results on 24 samples in 3 hours. Or do chemiluminescent and fluorescent multiplexing assays in parallel just to be absolutely sure you didn't miss a protein. Increase your sensitivity and multiplexing capabilities with neural samples using automated Western blotting systems. 2. FAX 612 656 4400. ", Brian Perrino, Ph.D., User Guide for Wes P/N 031-108 Revision 7, July 2018 For research use only. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple High-throughput pharmacokinetic analysis with quantitative, high-sensitivity assays directly in complex lysates and tissue homogenates. This protocol also describes treatment of glycosylated proteins with PNGase F. Dilute the antibodies in Antibody Diluent 2. Plus, use the Stellar Total Protein assay to normalize your multiplexed . Upon receipt, store it immediately at the temperature recommended below. Your sample, separation matrix, stacking matrix, antibodies, and reagents are loaded automatically from a specially designed plate. Jess gives you the qualitative Western blot data you are used to seeing. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. She separates your protein by size and precisely manages antibody additions, incubations, washes and even the detection steps. By continuing to use this website you acknowledge this and agree to our cookie policy. Validation was considered when differential expression at one or more . PDF User Guide for Jess - Bio-Techne Bio-Techne uses cookies to provide you with a great website experience. ", Elena Navarro-Guerrero, Ph.D., Minneapolis, MN 55413 Come back to fully analyzed and quantitated results in three hours. Jess performs Simple Western size-based assays: chemiluminescence or fluorescence immunoassays, and intra-assay protein normalization. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. Protein Simple- Jess. Analyze entire signaling pathways with high sensitivity multiplexing and total protein normalization. Simple Western assays are automated, capillary-based immunoassays that solve many of the challenges that come with traditional Westerns. Head of Research Instrumentation, Cancer Research UK Cambridge Institute, "Some of the proteins we wanted to detect are low in abundance and its really hard to get a good amount of protein for traditional Western blot. 3 0 obj Copyright 2023 Bio-Techne. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary. Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels. The throughput and system automation is a definite advantage. Go further with high sensitivitybest in class fluorescence detection gets all the information you need in one shot. With Jess, protein quantitation is a breeze. Meet Jess, your protein analysis problem solver. Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry, "The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-I and PKG-I and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies. PDF User Guide for Wes - Bio-Techne Just load your samples and reagents into the microplate and Jess does the rest. Senior Research Scientist, MiNA Therapeutics, London, UK, "Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps. For samples containing 0.2 1.2 mg/mL of protein, a fluorescent total protein assay can be run on Jess using the Fluorescent Protein Normalization Assay Module (see next section on Fluorescent Total Protein Assays). Or get info on protein abundance, isoform and size all at once. Multiple with chemluminescence & incandescence canal. FAX 612 656 4400. Jess automates traditional Western blotting while maximizing multiplexing with multiple detection channels. Simple Western can do the work for you. FAX 612 656 4400. Jess - Chemiluminescent & Fluorescent Western Blotting | ProteinSimple Jess, she's like Western blot meets ELISA in one. Jess from ProteinSimple, a Bio-Techne brand, automates and protein separation & immunodetection about traditional Western blotting. Question Asked 20th May, 2021 Ritishka Kapoor Florida International University Western blotting using #Jess #proteinsimple? Please note this module can only be used with Jess. A few months with Wes and we've already had way more success than we had in three years. ", Muhumad Noor Alfarizal Kamarudin, Using 5 times more concentrated Biotin Labeling Reagent, total protein detection using Simple Western can surpass the sensitivity of SDS-PAGE total protein stains including SYPRO Ruby. Minneapolis, MN 55413 Multiplex by chemiluminescence & fluorescence channels. ", Dr. Matteo Ferrari, Get the picture with her chemiluminescent blot imaging system. 2. Snap! Open Jess's door. After separation & immobilization of your sample proteins, the Protein Normalization Assay Module for Simple Western uses a proprietary molecule to fluorescently label proteins in your sample for detection. Frontiers | Methodologies to unlock the molecular expression and Western blotting using #Jess #proteinsimple? | ResearchGate Simply load your sample, antibodies and reagents into the plate, insert your plate and cartridge into Jess and press start. Want to identify whether a protein is present or absent? stream Got a lot of samples? Or detect high and low abundance proteins in the same sample, even when they have identical molecular weights. Quantitative results such as molecular weight, signal intensity (area), % area, and signal-to-noise for each immunodetected protein are presented in the results table automatically. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease", John K. Blackwood PhD, With Jess, get industry-leading sensitivity in chemiluminescence and fluorescence channels and the advantage of flexible multiplex analysis. Minneapolis, MN 55413 Toll-free 800 343 7475 Somebody have experience with the WES proteinsimple system? View all citations for the Simple Western platform. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. If you're more familiar with capillary electrophoresis, you can see your results in a more traditional electropherogram view too. I really like this system over traditional Western. Place the assay plate on the plate . Jess processes up to 25 samples in three hours, and Peggy Sue processes up to 96 samples overnight. Store the unopened product at room temperature. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. PDF Reprobe Your Immunoassay Samples Using Replex With Jess - With protein normalization, you can take your protein load comparison and transform your data to effectively normalize your samples, increasing your confidence in your data interpretation. "Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform. By continuing to use this website you acknowledge this and agree to our cookie policy. Jess automates the protein separation and immunodetection of traditional western blotting eliminating many of the tedious error-prone steps. The default protocols of the Jess system (ProteinSimple) were used for sized-based protein separation and detection in capillaries. Best of all, you get reliable and reproducible results with target normalization to the amount of protein loaded.
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